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The sample phase was yellow food coloring and the immiscible phase was light mineral oil with 0. A bulge of the digitized samples into the main channel was observed in some of our simulations. Using our chip it is possible to remove individual samples from the side cavities for downstream analysis either in full Figure 3a or in part Figure 3b. Pressure was applied until all of the sample cavities were filled and no air bubbles remained. We correlated this experimentally observed behavior with simulations using Fluent Figure 4e.

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Dynamic Sparse Sampling For Confocal Raman Microscopy

This observation has the potential to simplify the way in which a typical polymorph screen is conducted 35 , as well as significantly reduce the amount of material required to do so. Unless otherwise noted, the sample phase consisted of 1nM phosphate buffer. While this approach is successful in creating an array of spatially localized samples, filling the individual wells can be tedious. We investigated sample digitization in a series of forty different side chambers with various dimensions Figure 8 . see Supplementary Figure 4 for a list. Digitization did not occur in a freshly oxidized PDMS channel. The tubing connected to the sample phase inlet during filling was removed and the tubing connected to glass syringe was inserted in its place, taking care not to introduce any air bubbles into the system.

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Estimation of microquantities of cyanide

Our chip design with tall side chambers consisted of two inlets feeding into a main channel that was decorated with side cavities roughly twice the height of the main channel Figure 1a. The main channel was the same height as the side chambers. Raman spectra consistent with Form II were collected in all smaller volume chambers, except for some cavities with a volume below 2nL where the crystals melted during the Raman experiment and produced spectra of poor quality. We are interested in the utility of our digitization chip in these efforts. Interfacial Tension Interfacial tension is expected to be an important parameter in determining the digitization dynamics. The first criterion stems from the need for the aqueous sample phase to enter the chamber. if the chamber opening was too small in comparison with the cross-section of the main channel, the aqueous sample did not efficiently enter the chamber.

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Description: The final volume of the discretized sample at each location is defined by the geometry and size of each chamber. Single crystals in these smaller compartments visibly resembled Form II. The fluid flow profile and the volume fractions were then simulated with fractional steps for the pressure-velocity coupling. Figure 1i shows an array of side chambers. Without a surfactant, we found many of the cavities were left empty or partially filled. Images of digitized samples have been highlighted in bold. The PDMS-glass chips were exposed to oxygen plasma. A sample phase moving at faster rates never filled the sample cavity in its entirety Figure 4 left column and therefore the volume of the digitized sample decreased with increasing flow rate.
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